Fig. 2: Formation and reorganization of SNAP25:Syntaxin-1A binary complexes.
From: Mass spectrometry uncovers intermediates and off-pathway complexes for SNARE complex assembly
a SNAP25 and Syntaxin-1A were mixed in a 1:2 ratio and analysed by native MS. Charge state series corresponding to monomeric Syntaxin-1A (orange circle) and SNAP25 (green circle) as well as binary complexes composed of SNAP25 and Syntaxin-1A in a 1:1 (green-orange twin-circles), 1:2 (orange-green triangles) and 2:4 (orange-green stars) stoichiometry were observed. b Addition of Synaptobrevin-2 at a 1.2 molar excess to pre-assembled binary SNAP25:Syntaxin-1A complexes results in formation of the SNAP25:Syntaxin-1A:Synaptobrevin-2 (3Q:1 R) SNARE complex. Monomeric Synaptobrevin-2 (blue circles) and Syntaxin-1A (orange circles) as well as monomeric (grey triangles), dimeric (grey hexagonal stars) and trimeric (grey nonagonal stars) SNARE complexes are assigned. The observed molecular weight is given for all proteins and complexes (see legend).
