Fig. 3: Formation and rearrangement of the SNAP25:Synaptobrevin-2 binary complex.
From: Mass spectrometry uncovers intermediates and off-pathway complexes for SNARE complex assembly
a SNAP25 and Synaptobrevin-2 were mixed in a 1:1 ratio and analysed by native MS. Charge state series corresponding to monomeric Synaptobrevin-2 (blue circle) and SNAP25 (green circle), dimeric SNAP25 (green twin-circles) as well as binary complexes composed of SNAP25 and Synaptobrevin-2 with a stoichiometry of 2:1 (green-blue triangle) and 4:2 (green-blue hexagonal stars) were observed. b Upon equimolar addition of Syntaxin-1A, monomeric (grey triangles), dimeric (grey hexagonal stars), trimeric (grey nonagonal stars) and tetrameric (grey dodecagonal) SNARE complexes were observed. The observed molecular weight is given for all proteins and complexes (see legend).
