Fig. 6: MiR-525-5p directly targets heme oxygenase 1.
From: CircFNDC3B regulates osteoarthritis and oxidative stress by targeting miR-525-5p/HO-1 axis
a The target mRNAs of miR-525-5p were predicted by miRDB, Targetscan, PITA and Osteoarthritis-related genes. The diagram demonstrated the overlapping results. b RT-qPCR analysis of 8 mRNA candidates in the HCs lysates. (n = 3). c Schematic illustration showing the predicted binding region of miR-525-5p and HO-1. d Upper panel, schematic illustration demonstrates complementary to the miR-525-5p seed sequence with HO-1. Lowercase letters indicate mutated nucleotides. Lower panel, HEK-293T cells were co-transfected with miR-145-5p mimics and a luciferase reporter construct containing wild-type (WT) or mutated HO-1. (n = 3). e, f Western blot and RT-qPCR analysis of HO-1 in HCs after treated with si-CircFNDC3B or miR-525-5p mimic. (n = 3). Mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001 by Student’s t test.
