Fig. 1: RAGA regulates lung adenocarcinoma progression and macrophage phagocytosis.
From: RAGA prevents tumor immune evasion of LUAD by promoting CD47 lysosome degradation
a Immunoblot analysis of indicated proteins in control and RAGA knockdown A549 cells. b Relative cell number of control and RAGA knockdown A549 cells (n = 3 per group). c Representative images of control and RAGA knockdown A549 colonies. d Relative colony number of control and RAGA knockdown A549 cells (n = 3 per group). e The tumor xenografts of control and RAGA knockdown A549 cells. f The growth curve of control and RAGA knockdown A549 tumor xenografts (n = 5 per group). g The weight of control and RAGA knockdown A549 tumor xenografts (n = 5 per group). h Representative flow cytometry analysis of in vivo phagocytosis in tumor xenografts. Numbers in the upper panel outlined areas indicate the percentage of tumor macrophages. Numbers in the lower panel outlined areas indicate the percentage of tumor macrophages with phagocytosis. i Statistical analysis of the percentage of macrophages with phagocytosis in control and RAGA knockdown A549 tumor xenografts (n = 4 per group). j Representative flow cytometry analysis of in vitro phagocytosis in macrophage and GFP-labeled A549 coculture system. Numbers in the outlined areas indicate the percentage of macrophages with phagocytosis. k Statistical analysis of the percentage of macrophages with phagocytosis in the coculture system as in (j) (n = 4 per group). Data were from two independent experiments. Statistical data were presented as mean ± SD. P < 0.05 is considered statistical significance. ** indicates P < 0.01; **** indicates P < 0.0001; ns indicates not significant.
