Fig. 2: RAGA depletion suppresses phagocytosis and promotes tumor growth via CD47 accumulation.
From: RAGA prevents tumor immune evasion of LUAD by promoting CD47 lysosome degradation
a Immunoblot analysis of indicated proteins in control and RAGA knockdown A549 cells. b Representative flow cytometry of CD47 protein expression on the cell surface. c Statistical analysis of cell surface CD47 expression (n = 4 per group). d Representative flow cytometry analysis of in vitro phagocytosis in macrophage and GFP-labeled A549 coculture system. Numbers in the outlined areas indicate the percentage of macrophages with phagocytosis. e Statistical analysis of the percentage of phagocytic macrophages cocultured with control and RAGA knockdown A549 cells under IGG or anti-CD47 antibody treatment (n = 4 per group). f The tumor xenografts of control and RAGA knockdown A549 with IGG or anti-CD47 antibody treatment. g The growth curve of A549 tumor xenografts is shown in (f) (n = 5 per group). h The weight of A549 tumor xenografts is shown in (f) (n = 5 per group). Data were from two or three independent experiments. Statistical data were presented as mean ± SD. P < 0.05 is considered statistical significance. *indicates P < 0.05 and **** indicates P < 0.0001.
