Fig. 4: RAGA promotes CD47 lysosome degradation.
From: RAGA prevents tumor immune evasion of LUAD by promoting CD47 lysosome degradation
a Immunofluorescence staining of CD47 and LAMP1 in HBE, A549, and H1299 cells. The yellow arrow indicates cell surface CD47 staining. The white arrow indicates the colocalization of CD47 with LAMP1. b Statistical analysis of PM/intracellular CD47 expression ratio (n = 10 cells per group). c Statistical analysis of the percentage of CD47 colocalized with LAMP1 (n = 10 image areas per group). d Immunoblot analysis of the indicated proteins in 100 μM cycloheximide (CHX) treated A549 cells. h indicates hours. e Immunoblot analysis of the indicated proteins in A549 cells treated with different dosages of bafilomycin A1. f Immunoblot analysis of the indicated proteins in A549 cells treated with different dosages of chloroquine. g Immunofluorescence staining of CD47 and RAGA in HBE and A549 cells. The white arrow indicates the colocalization of CD47 with RAGA. h Immunoblot analysis of indicated proteins assessed after RAGA immunoprecipitation. i Immunoblot analysis of indicated proteins in control and RAGA knockdown A549 cells treated with or without bafilomycin A1. Bafi indicates bafilomycin A1. Data were from two independent experiments. Statistical data were presented as mean ± SD. P < 0.05 is considered statistical significance. **** indicates P < 0.0001.
