Fig. 5: High-throughput screening in sporozoite viability assays. | Communications Biology

Fig. 5: High-throughput screening in sporozoite viability assays.

From: Assessment of the drugability of initial malaria infection through miniaturized sporozoite assays and high-throughput screening

Fig. 5

a Schematic representation of the high-throughput screenings carried out with the GHCDL and the reFRAME library with our luminescence-based sporozoite viability assay and luciferase interference assay. b Viability of sporozoites after 24-h incubation (red dots) and recombinant luciferase signal after a 1-h incubation (blue dots) with different doses of the hits from the GHCDL and reFRAME library. Activity is expressed as the percentage of the positive and negative control luminescence signal. The figure shows data from two replicate experiments. c Quantification of the gliding trails of sporozoites treated with a single 10 µM and 3.3 µM dose of hit compounds from the GHCDL (DDD compound codes) and reFRAME (CBR compounds) library, respectively. Error bars indicate standard deviations from two replicates, which are shown by individual symbols.

Back to article page