Fig. 3: Improved insulin sensitivity and reduced insulin secretion in obese REG3A transgenic mice fed a high fat diet. | Communications Biology

Fig. 3: Improved insulin sensitivity and reduced insulin secretion in obese REG3A transgenic mice fed a high fat diet.

From: Antimicrobial protein REG3A regulates glucose homeostasis and insulin resistance in obese diabetic mice

Fig. 3

Four-month-old REG3A transgenic mice (REG3A) and wild-type (WT) mice (n = 8 REG3A, n = 9 WT, new cohorts different from Figs. 1 and 2) were fed a high-fat, butter-enriched diet (5505 kcal/kg) for 8 weeks and then analyzed. a cumulative food intake. b Body weight gain. c Blood glucose curves under oral glucose tolerance tests OGTT. Right: area under the curve of OGTT. d–f Blood tests (d) Blood insulin, e C-peptide concentrations and f Insulin resistance indices (HOMA-IR). g Blood glucose curves under insulin tolerance tests ITT. Right: area under the curve of ITT assays. (n = 7 per group). h Representative images of insulin staining on pancreas sections from REG3A transgenic and WT mice fed standard (CD) or a high-fat (HFD) diet, and quantification of insulin positive areas. Scale bar: 200 µm. Data are averages ± SEM. ∗p < 0.05 by Anova test (d, e, h), one-way repeated measures Anova (a, b, left c, left g) both followed by post-hoc test, Wilcoxon rank sum test (right c, right g). NS or no statistical indication, no significance.

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