Fig. 1: SETD2 deficiency promotes OXPHOS and protein synthesis gene expression programs in human and murine lung adenocarcinomas.

a Gene set enrichment analysis (GSEA) network plot of the 20 most enriched gene sets negatively-correlated with SETD2 expression in human lung adenocarcinomas. The size of each node corresponds to the normalized enrichment score (NES) and the width of the connecting lines indicates the number of overlapping genes between gene sets. Gene sets are categorized as mitochondrial, ribosomal or other according to the genes represented. b GSEA network plot of the top 20 enriched gene sets in KPY-Setd2^KO lung adenocarcinomas. The size of each node corresponds to the normalized enrichment score (NES) and the width of the connecting lines indicates the number of overlapping genes between gene sets. Gene sets are categorized as mitochondrial, ribosomal or other according to the genes represented. c Representative mitochondrial (GO Electron Transport Chain) and ribosomal (GO Cytoplasmic Translation) GO biological process ontology gene sets that are enriched in K-Setd2^KO (left) and KPY-Setd2^KO (right) tumors. d Depiction of mitochondrial electron transport chain genes that show enrichment in the molecular signatures database (mSigDB) Mootha VOXPHOS gene set in K-Setd2^KO tumors⁵³. Genes are grouped according to the relevant ETC complex. Genes that are part of the core enrichment of the gene set are marked in gray, genes that are not enriched are marked in white, and mitochondrial encoded genes are outlined in red. e Depiction of ribosomal 40 S and 60 S genes that show enrichment in the mSigDB GO Ribosome Biogenesis gene set in K-Setd2^KO tumors. Genes that are enriched in this gene set are marked in gray while genes that are not enriched are marked in white.