Fig. 4: UVA upregulates the expression of Dync1i1 and DCTN1 via OPN3.
From: Opsin 3 mediates UVA-induced keratinocyte supranuclear melanin cap formation
a HaCaT was transfected with lentivirus shOPN3 (shOPN3#1) and control lentivirus (shNC), and after irradiated without or with UVA, WB was used to analyze changes of OPN3, Dync1i1, and DCTN1 protein expression levels in HaCaT (n = 3 independent experiments). WB analyses were normalized using β-tubulin as a loading control, and the relative protein level was quantified using Quantity One software. Statistical significance was determined by one-ANOVA with post-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. b–d After shOPN3 inhibited OPN3 irradiated without or with UVA, RT-qPCR was used to analyze changes of OPN3, Dync1i1, and DCTN1 mRNA expression in HaCaT (n = 3 independent experiments). Statistical significance was determined by one-ANOVA with post-test. **P < 0.01, ***P < 0.001, ****P < 0.0001. e HaCaT was transfected with lentivirus overexpression OPN3 (LV-OPN3) and control lentivirus (LV-control) and irradiated without or with UVA. Then, WB was used to analyze changes in OPN3, Dync1i1, and DCTN1 expression in HaCaT. β-tubulin was used as a loading control. Statistical significance was determined by one-ANOVA with post-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. f–h After LV-OPN3 overexpression of OPN3 irradiated without or with UVA, RT-qPCR was used to analyze changes in OPN3, Dync1i1, and DCTN1 expression levels in HaCaT. OPN3, Dync1i1, and DCTN1 mRNA levels were normalized to GAPDH levels (n = 3 independent experiments). Statistical significance was determined by one-ANOVA with post-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
