Fig. 7: OPN3 regulates the expression of Dync1i1 and DCTN1 via the AKT signaling pathway.
From: Opsin 3 mediates UVA-induced keratinocyte supranuclear melanin cap formation
a Cells were irradiated without and with UVA, and AKT protein and phosphorylated-AKT (p-AKT) protein levels were determined by WB. β-tubulin was used as a loading control. The relative protein level was quantified using Quantity One software. Statistical significance was determined by t-test analysis. **P < 0.01. b HEK were transfected with siRNA against OPN3 irradiated with UVA and lysed after 1 h. Lysates were analyzed by WB using the indicated antibodies (anti-OPN3, anti-AKT, anti-p-AKT, anti-Dync1i1, and anti-DCTN1). β-tubulin was used as a loading control. The relative protein level was quantified using Quantity One software. Statistical significance was determined by one-ANOVA with post-test. ***P < 0.001. c HEK treated with U73122 were stimulated with 3 J/cm2 UVA, and protein lysate was analyzed by WB. β-tubulin was used as a loading control. The relative protein level was quantified using Quantity One software. Statistical significance was determined by one-ANOVA with post-test. ***P < 0.001. d HEK treated with MK-2206 (2HCl) were stimulated with 3 J cm−2 UVA, and protein lysate was analyzed by WB. β-tubulin was used as a loading control. The relative protein level was quantified using Quantity One software. Statistical significance was determined by one-ANOVA with post-test. **P < 0.01, ***P < 0.001.
