Fig. 2: Melanin synthesis is decreased in Mewo-Z5Z7-DKO cells. | Communications Biology

Fig. 2: Melanin synthesis is decreased in Mewo-Z5Z7-DKO cells.

From: Pigmentation and TYRP1 expression are mediated by zinc through the early secretory pathway-resident ZNT proteins

Fig. 2

a Pellets of wild-type (WT) Mewo and Mewo-Z5Z7-DKO cells, and of Mewo-Z5Z7-DKO cells stably re-expressing ZNT7-HA. b Melanin content was decreased in Mewo-Z5Z7-DKO cells compared with that in WT Mewo cells; the decrease was recovered upon expression of ZNT7 (n = 3). Statistical significance was determined using the one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. **p < 0.01. c Transmission electron microscopy (TEM) showing irregular melanosomes with less electron dense pigment in Mewo-Z5Z7-DKO cells compared with those in WT Mewo cells. Each experiment, except for that in c, was performed at least three times, and representative results from independent experiments are shown. Panels in c show representative results for at least three cells.

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