Fig. 4: Mewo-Z5Z7-DKO cells show similar defects as in Mewo-TYRP1-KO cells. | Communications Biology

Fig. 4: Mewo-Z5Z7-DKO cells show similar defects as in Mewo-TYRP1-KO cells.

From: Pigmentation and TYRP1 expression are mediated by zinc through the early secretory pathway-resident ZNT proteins

Fig. 4

a Analysis of TYRP1, TYR, and TYR2 expression by immunoblotting in wild-type (WT) Mewo, Mewo-Z5Z7-DKO and Mewo-TYRP1-KO cells. Note the loss of TYRP1, but not TYR and TYRP2, in both Mewo-Z5Z7-DKO and Mewo-TYRP1-KO cells. b Immunofluorescence analysis confirmed a loss of TYRP1 in both Mewo-Z5Z7-DKO and Mewo-TYRP1-KO cells. c Pellets of Mewo-Z5Z7-DKO and Mewo-TYRP1-KO cells exhibit hypopigmentation compared to WT Mewo cells. d Melanin content was decreased in Mewo-TYRP1-KO cells, similar to that in Mewo-Z5Z7-DKO cells (n = 3). Statistical significance was determined using the one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. **p < 0.01. e TEM showed irregular melanosomes with less electron dense contents in Mewo-TYRP1-KO, compared with WT Mewo cells. Each experiment except for (e) was performed at least three times, and representative results from independent experiments are shown. Panels in e were performed using at least three cells, and representative results are shown.

Back to article page