Fig. 6: Restoration of mouse or chicken Tyrp1 expression in SK-Z5Z7-DKO cells following re-expression of mouse or chicken Znt5 or Znt7. | Communications Biology

Fig. 6: Restoration of mouse or chicken Tyrp1 expression in SK-Z5Z7-DKO cells following re-expression of mouse or chicken Znt5 or Znt7.

From: Pigmentation and TYRP1 expression are mediated by zinc through the early secretory pathway-resident ZNT proteins

Fig. 6

a, b Expression of recombinant mouse (a) and chicken (b) Tyrp1 was substantially decreased in SK-Z5Z7-DKO cells compared to wild-type (WT) SK-MEL-2 cells, but stabilized by expression of recombinant mouse or chicken Znt5 or Znt7. In b, cTyrp1, tagged with HA at the C-terminus, was used. c Immunofluorescence staining of mouse Tyrp1 expressed in WT SK-MEL-2 or SK-Z5Z7-DKO cells. Tyrp1 and Znt plasmids were transfected at a ratio of 1:10. Each experiment was performed at least three times, and representative results from independent experiments are shown.

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