Fig. 4: Validation of S100A8, S100A9 protein expression in TS plaques and human carotid plaques. | Communications Biology

Fig. 4: Validation of S100A8, S100A9 protein expression in TS plaques and human carotid plaques.

From: Quantitative proteomic landscape of unstable atherosclerosis identifies molecular signatures and therapeutic targets for plaque stabilization

Fig. 4

a S100A9, MPO, and DAPI immunofluorescence staining in unstable TS mice plaques (n = 10) and b stable plaques (n = 10). As a negative control, c IgG Isotype control antibodies for S100A9 and MPO were deployed. d Western blots were performed in three TS mice, and each artery segment was collected for protein identification. S100A9 is only found in the segments that are unstable. e S100A8 and g S100A9 immunohistochemistry in human carotid plaques (n = 14). As a negative control, IgG Isotype control antibodies for f S100A8 and h S100A9 were deployed. Bars indicate 100 μm. H/E: Hematoxylin and eosin, L: Lumen, Ath: Atherosclerosis. Dashed lines indicate endothelium.

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