Fig. 2: Knockdown of LFPRLR in SLE-prone mice decreases factors associated with the risk of lymphoma initiation. | Communications Biology

Fig. 2: Knockdown of LFPRLR in SLE-prone mice decreases factors associated with the risk of lymphoma initiation.

From: Isoform-specific knockdown of long and intermediate prolactin receptors interferes with evolution of B-cell neoplasms

Fig. 2

a–d Verification of LFPRLR knockdown in isolated B cells by qPCR (a), percentages of B cells in sub G0/G1, G0/G1, S, and G2/M phases with representative flow cytometry plots (b), B-cell specific transcript levels of Myc, Bcl2 by qPCR (c), and BCL2 protein by flow cytometry (d) in spleens of MRL-lpr mice treated with control SMO (n = 14, red) or LFPRLR SMO (n = 14, blue). e–g Next-generation sequencing of total IGH repertoire (GSE207186) to compare spectratype of splenic B cells (e), and frequencies of splenic B cells with IGH CDR3 ≥ 20aa (f), and frequencies of B cells with non-productive IGH rearrangements (g) in MRL-lpr SLE-prone mice treated with control (n = 6, red) or LFPRLR SMO (n = 6, blue). h Aicda mRNA and protein levels in splenic B cells and representative histograms for AID protein staining in control SMO-treated (n = 14, red) and LFPRLR SMO-treated (n = 14, blue) MRL-lpr mice by flow cytometry. Splenic WBCs from wildtype mice cultured with IL-4 and LPS were used as positive controls for AID staining. Each dot in qPCR analyses of transcripts corresponds to the mean expression of that gene in one mouse calculated from 3 technical replicates. Ubb was used as the house-keeping gene in qPCR. Graphs show median ± interquartile range. Exact p-values were calculated using the Mann–Whitney U test. ns non-significant, FMO fluorescence minus one control, MFI median fluorescence intensity.

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