Fig. 1: Embryonic lethality of whole-body Nsun3 KO mice. | Communications Biology

Fig. 1: Embryonic lethality of whole-body Nsun3 KO mice.

From: NSUN3-mediated mitochondrial tRNA 5-formylcytidine modification is essential for embryonic development and respiratory complexes in mice

Fig. 1

a Secondary structure of the mouse mitochondrial (mt-) tRNAMet with modified nucleosides: pseudouridine (Ψ) and 5-formylcytidine (f5C). The modifications are depicted based on human and bovine mt-tRNAMet modifications7,50. The nucleoside position is numbered following conventional guidelines51. Note that f5CAU anticodon can base pair with two mitochondrial methionine-encoding mRNA codons AUG and AUA. b Chemical structure of f5C. The formyl modification at the cytidine C5 position is shown in red. c Numbers of animals obtained by crossing parental heterozygous (Nsun3+/−) mice. P value was calculated by the chi-square test. d Genotyping analysis of embryos at stage E12.5. e, f Morphology of WT (+/+), heterozygous (+/−), and KO (−/−) embryos at stages E12.5 (e) and E10.5 (f) removed from the uterus of a heterozygous mother mouse. Scale bars, 5 mm (e) and 1 mm (f).

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