Fig. 2: At steady state, PIP2 signals are more abundant in dendritic spines than in dendrites and occur in particular in spine heads. | Communications Biology

Fig. 2: At steady state, PIP2 signals are more abundant in dendritic spines than in dendrites and occur in particular in spine heads.

From: Long-term depression in neurons involves temporal and ultra-structural dynamics of phosphatidylinositol-4,5-bisphosphate relying on PIP5K, PTEN and PLC

Fig. 2

a–c TEM images (a, b) of an anti-PIP2 immunogold-labeled dendritic spine of a freeze-fractured hippocampal neuron (DIV16) with outlined spine and transparent coloring of dendrite (blue), spine base (green), spine neck (yellow), and spine head (red) (a), as depicted schematically in c, and corresponding raw TEM image (b). Bars, 200 nm. d, e Quantitative analyses of anti-PIP2 immunogold labeling densities of dendritic versus dendritic spine plasma membrane areas (d) and of subspine areas (e), respectively. Data, mean ± SEM of 16 independent assays; n = 159 ROIs each (dendrite, total spine, spine base, neck, and head). Statistical significance calculations, Mann–Whitney (d) and Kruskal–Wallis/Dunn’s (e). **P < 0.01; ****P < 0.0001. For P < 0.0001, exact P-values are not available. The other P-value is reported in the figure. For numerical source data, see Supplementary Data 1.

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