Fig. 1: S. aureus aggregates in pSynF are metabolically suppressed and exhibit tolerance to amikacin. | Communications Biology

Fig. 1: S. aureus aggregates in pSynF are metabolically suppressed and exhibit tolerance to amikacin.

From: Microbubble cavitation restores Staphylococcus aureus antibiotic susceptibility in vitro and in a septic arthritis model

Fig. 1

a Scanning electron micrographs of an S. aureus aggregate formed in a model SynF, i.e., pSynF. Successive magnifications are indicated and highlight the bacteria covered in matrix. b Alamar blue fluorescence as an indicator of S. aureus metabolic activity in TSB and pSynF (inset shows an expansion of the y-axis). Note the >10× suppression in metabolic activity in pSynF. c S. aureus sensitivity to 30 µg/mL of AMK as a function of initial inoculum and of culturing in TSB or pSynF. Notably, bacterial survival is apparent even at the lowest inoculum when cultured in pSynF. Data presented as mean ± standard deviation. For each point b or bar c, n = 18. Individual data points are shown as open, colored symbols. Normality checked using Kolmogorov–Smirnov test. Comparisons done using two-tailed Wilcoxon rank sum test. ****P < 0.0001.

Back to article page