Fig. 3: Dilution and water bath sonication increase MSSA AMK sensitivity. | Communications Biology

Fig. 3: Dilution and water bath sonication increase MSSA AMK sensitivity.

From: Microbubble cavitation restores Staphylococcus aureus antibiotic susceptibility in vitro and in a septic arthritis model

Fig. 3

a The effects of increasing dilution on aggregate dispersal, where dilution with PBS (changing volume, changing protein/viscosity), pSynF (changing volume, constant viscosity), and PBS (constant volume, changing protein/viscosity) are indicated. b Changes in metabolic activity of S. aureus in 20% TSB and 20% pSynF with aggregated S. aureus, as indicated by Alamar blue fluorescence after bath sonication. c The effect of dilution and 30 min sonication on AMK sensitivity of MSSA in TSB, pSynF, and SynF. Data are presented as mean ± standard deviation. For each bar a, n = 16 (changing volume, changing viscosity; pSynF, 100%, 30 min) or 18, b, c, n = 12. Individual data points are shown as open, colored symbols. Normality was checked using Kolmogorov–Smirnov test. Comparisons were done using a Kruskal–Wallis test for multiple comparisons or (b, c) Mann–Whitney tests for paired comparison on the basis of independent groups. ns, P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

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