Fig. 2: GIL-11 induce JAK/STAT signaling and cellular proliferation via the non-natural gp130:IL-11R:LIFR cytokine receptor complex. | Communications Biology

Fig. 2: GIL-11 induce JAK/STAT signaling and cellular proliferation via the non-natural gp130:IL-11R:LIFR cytokine receptor complex.

From: Cytokimera GIL-11 rescued IL-6R deficient mice from partial hepatectomy-induced death by signaling via non-natural gp130:LIFR:IL-11R complexes

Fig. 2

a Proliferation of Ba/F3-gp130, Ba/F3-gp130-IL-11R, Ba/F3-gp130:LIFR, Ba/F3-gp130:OSMR, Ba/F3-gp130:IL-11R:OSMR, Ba/F3-gp130:IL-6R:LIFR, Ba/F3-gp130:LIFR:IL-11R cells without cytokine (-), with 50 ng/ml HIL-11, 50 ng/ml IL-11, 50 ng/ml IL-6, 10 ng/ml LIF, 10 ng/ml OSM, 500 ng/ml GIL-11. Data represent ±SEM of three independent experiment out of three with three technical replicates each. Two-way ANOVA, including Dunnet’s correction was used for statistics. b STAT3 activation in Ba/F3, Ba/F3-gp130, Ba/F3-gp130_IL-11R, Ba/F3-gp130:LIFR, Ba/F3-gp130:OSMR, Ba/F3-gp130:IL-11R:OSMR, Ba/F3-gp130:IL-6R:LIFR, Ba/F3-gp130:LIFR:IL-11R cells without cytokine (-) and after stimulation with 50 ng/ml HIL-11, 50 ng/ml IL-11, 10 ng/ml LIF, 10 ng/ml OSM, 500 ng/ml GIL-11 for 15 min. Equal amounts of proteins (50 µg/lane) were analyzed via specific antibodies detecting phospho-STAT3 and STAT3. Western blot data shows one representative experiment out of three.

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