Fig. 5: GIL-11:sIL-11 complexes induce trans-signaling via gp130:LIFR. | Communications Biology

Fig. 5: GIL-11:sIL-11 complexes induce trans-signaling via gp130:LIFR.

From: Cytokimera GIL-11 rescued IL-6R deficient mice from partial hepatectomy-induced death by signaling via non-natural gp130:LIFR:IL-11R complexes

Fig. 5

a Proliferation of Ba/F3-gp130:LIFR cells in the presence and absence of fixed concentrations of sIL-11R (0 or 100 ng/ml) and increasing concentrations of IL-11 (1–2000 ng/ml). One representative experiment out of three is shown. b Proliferation of Ba/F3-gp130:LIFR cells in the presence and absence of fixed concentrations of sIL-11R (0 or 100 ng/ml) and increasing concentrations of GIL-11 (1–2000 ng/ml). One representative experiment out of three is shown. c STAT3 activation in Ba/F3-gp130:LIFR cells without cytokine (-) and after stimulation with HIL-11 (50 ng/ml), GIL-11 (1 µg/ml), GIL-11 (1 µg/ml):sIL-11R (2 µg/ml), GIL-11 (1 µg/ml):sIL-11R (2 µg/ml):sgp130Fc (10 µg/ml) for 15 min. Equal amounts of proteins (50 µg/lane) were analyzed via specific antibodies detecting phospho-STAT3 and STAT3. Western blot data shows one representative experiment out of three. d Proliferation of Ba/F3-gp130:LIFR cells in the presence and absence of fixed concentrations of IL-11 (0.5 µg/ml):sIL-11R (1 µg/ml) or GIL-11 (0.5 µg/ml):sIL-11R (1 µg/ml) and increasing concentrations of sgp130Fc (1–10,000 ng/ml). One representative experiment out of three is shown. e Proliferation of Ba/F3-gp130:LIFR cells in the presence and absence of fixed concentrations of LIF (10 ng/ml) and increasing concentrations of GIL-11 (1–2000 ng/ml) (green). Proliferation of Ba/F3-gp130:IL-11R cells in the presence and absence of fixed concentrations of IL-11 (100 ng/ml) and increasing concentrations of GIL-11 (1–2000 ng/ml) (red). Error bars define ±SEM. One representative experiment with three biological replicates out of three is shown.

Back to article page