Fig. 2: Light-sheet fluorescence microscopy (LSFM) with OptoRheo.
From: OptoRheo: Simultaneous in situ micro-mechanical sensing and imaging of live 3D biological systems
a Schematic of light-sheet microscopy on the OptoRheo. Sample consists of cell clusters (represented by red circles with blue centres) and collagen (purple lines). b Multicolour imaging of a co-culture of MDA-MB-231 (top left corner of montage) and MCF-7 (top right corner) in hydrogel supplemented with Cy-5 labelled collagen I (bottom left corner) on the OptoRheo with three colour channels. Images in montage are maximum intensity projections. Scale bar = 20 µm. c Single planes from time lapse movie (Supplementary Video SV1) taken on the OptoRheo of a MDA-MB-231 cell cluster changing morphology. Scale bar = 10 µm. d 3D rendering of b.
