Fig. 2: Retention of binding capacities of antibodies after conjugation to WGA.
From: Extending the use of biologics to mucous membranes by attachment of a binding domain
a Binding of ligands to WGA-conjugated antibodies. GlcNAc agarose beads were incubated with the indicated antibodies and subsequently with fluorescently labeled ligands and photographed with a fluorescence microscope. Binding does not occur to the WGA anchor (cf. Supplementary Fig. 4). Quadruplicate values ± SE. b Specificity of binding to GlcNAc. Anti-IL-1β antibodies were incubated with GlcNAc agarose beads in the presence of 0.2 M of the indicated competitors. After washing, they were incubated with fluorescently labeled IL-1β as indicated. Similar experiments with additional antibody conjugates are shown in Supplementary Fig. 2. The orange scale bar represents 0.1 cm.
