Fig. 3: Retention of IL-17A antibody on the ocular surface of mice operated to induce dry eye.
From: Extending the use of biologics to mucous membranes by attachment of a binding domain

a Retention of fluorescently labeled IL-17A antibody. Alexa Fluor 488-labeled antibodies were applied to eyes and followed by fluorescence imaging. Quadruplicate determinations, means ± SE. b, c Histological analysis of applied antibody (Ab) in tissues. The anti-IL-17A antibody was raised in a rat host and was detected with an Alexa Fluor 555 labeled anti-rat IgG antibody (bright orange) (b) IL-17A antibody was applied to eyes and they were harvested 5 min later. The eyes were treated with no antibody, antibody without anchor, or antibody with anchor, as indicated. The specificity of the signals was verified by preincubating the anti-rat IgG with the anti-IL-17A antibody (“+Competitor”). The epithelium was visualized by staining with phalloidin, which stains f-actin (green). The overlay is a composite of the anti-IL-17A antibody with anchor condition stained with anti-IgG and with phalloidin, and the insert in is a higher magnification of a section of the epithelium showing presence of antibody in the mucin layer (yellow). c As (b) except the mice were harvested 24 h after application of anti-IL17A antibody and a longer exposure time for photography was used. Antibody is seen in the lower part of the epithelium and in the anterior part of the underlying stroma in some regions. The orange scale bars represent 100 µm.