Fig. 5: The mechanical stimulation can induce the changes of intracellular chloride through Ano1 in osteoclast. | Communications Biology

Fig. 5: The mechanical stimulation can induce the changes of intracellular chloride through Ano1 in osteoclast.

From: Mechanical stimulation controls osteoclast function through the regulation of Ca2+-activated Cl− channel Anoctamin 1

Fig. 5

Measurement of intracellular chloride concentration in osteoclasts. All the cells were stained with 5 mM MQAE. a Representative fluorescent images of Ano1fl/fl and Ctsk-Cre;Ano1fl/fl osteoclasts after treatment with Ctrl or FSS (12 dyn/cm2, 30 min/day) for 2 days (left). Scale bar, 100 μm. The relative fluorescence intensity of osteoclasts (right). (n = 3 independent experiments). b Representative fluorescent images of Ano1fl/fl and Ctsk-Cre;Ano1fl/fl osteoclasts after treatment with Ctrl or HG (4 g) for 2 days (left). Scale bar, 100 μm.The relative fluorescence intensity of osteoclasts (right). (n = 3 independent experiments). c Representative fluorescent images of Ano1fl/fl and Ctsk-Cre;Ano1fl/fl osteoclasts after treatment with Ctrl or MG for 2 days (left). Scale bar, 100 μm. The relative fluorescence intensity of osteoclasts (right). (n = 3 independent experiments). All data are the mean ± s.e.m. Statistical analysis with more than two groups was performed with two-way analysis of variance (ANOVA) with Šídák post-hoc test to determine group differences. **p < 0.01, ***p < 0.001.

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