Fig. 6: Ano1 regulates calcium activated signaling in response to mechanical stimulation in osteoclast. | Communications Biology

Fig. 6: Ano1 regulates calcium activated signaling in response to mechanical stimulation in osteoclast.

From: Mechanical stimulation controls osteoclast function through the regulation of Ca2+-activated Cl− channel Anoctamin 1

Fig. 6

a Western blot analysis of p-CaMKIV, CaMKIV, p-Creb,Creb and NFATc1 protein levels in Ano1fl/fl and Ctsk-Cre;Ano1fl/fl osteoclasts with Ctrl or FSS (12 dyn/cm2, 30 min/day) treatment for 2 days. b–d The quantification of p-CaMKIV, p-Creb and NFATc1 protein levels in osteoclasts. The p-CaMKIV protein level was normalized to CaMKIV, the p-Creb protein level was normalized to Creb, and the NFATc1 protein level was normalized to Gapdh. (n = 3 independent experiments). e Western blot analysis of p-CaMKIV, CaMKIV, p-Creb,Creb and NFATc1 protein levels in Ano1fl/fl and Ctsk-Cre;Ano1fl/fl osteoclasts with Ctrl or HG (4 g) treatment for 2 days. f–h The quantification of p-CaMKIV, p-Creb and NFATc1 protein levels in osteoclasts. The p-CaMKIV protein level was normalized to CaMKIV, the p-Creb protein level was normalized to Creb, and the NFATc1 protein level was normalized to Gapdh. (n = 3 independent experiments). i Western blot analysis of p-CaMKIV, CaMKIV, p-Creb,Creb and NFATc1 protein levels in Ano1fl/fl and Ctsk-Cre;Ano1fl/fl osteoclasts with Ctrl or MG treatment for 2 days. j–l The quantification of p-CaMKIV, p-Creb and NFATc1 protein levels in osteoclasts. The p-CaMKIV protein level was normalized to CaMKIV, the p-Creb protein level was normalized to Creb, and the NFATc1 protein level was normalized to Gapdh. (n = 3 independent experiments). All data are the mean ± s.e.m. Statistical analysis was performed with two-way analysis of variance (ANOVA) with Šídák post-hoc test to determine group differences. *p < 0.05, **p < 0.01, ***p < 0.001.

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