Fig. 7: Osteoclast specific Ano1 knock out protects from unloading induced bone loss.
a, b QRT-PCR analysis of Ano1 mRNA level and western blot analysis of Ano1 protein level in bone tissues from Ano1fl/fl and Ctsk-Cre;Ano1fl/fl mice with Ctrl or hind limb suspension (HS) treatment. (n = 6 for each group). c Representative images showing three-dimensional trabecular architecture as determined by micro-CT reconstruction of the distal femurs from the groups of mice indicated. Scale bar, 0.5 mm. d Micro-CT measurements for bone volume per tissue volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th) and trabecular spacing (Tb.Sp) in the distal femurs from the groups of mice indicated. (n = 6 for each group). e Representative images of TRAP staining of the proximal tibia from the groups of mice indicated. Scale bar, 50 μm. f Histomorphometry analysis of the images for number of osteoclasts per bone perimeter (N.Oc/B.Pm) and osteoclast surface per bone surface (Oc.S/BS). (n = 6 for each group). g ELISA analysis of CTX-1 protein level in serum from the groups of mice indicated. (n = 6 for each group). h QRT-PCR analysis of NFATc1, Acp5, Ctsk and Mmp9 mRNA levels in bone tissues collected from the groups of mice indicated. (n = 6 for each group). Statistical analysis with more than two groups was performed with two-way analysis of variance (ANOVA) with Šídák post-hoc test to determine group differences. *p < 0.05, **p < 0.01, ***p < 0.001.
