Table 1 The fluorophore-labeled single-stranded DNA probes used in this study.
From: RT-IVT method allows multiplex real-time quantification of in vitro transcriptional mRNA production
Probe | Sequence& Fluorescent mark | |
|---|---|---|
BFQ1 | BFQ1a2 | 5′-pT/FAM/TTTTGGATGGTGGTTGACGGCG-3′ |
BFQ1a3 | 5′-pTT/FAM/TTTGGATGGTGGTTGACGGCG-3′ | |
BFQ1a4 | 5′-pTTT/FAM/TTGGATGGTGGTTGACGGCG-3′ | |
BFQ1a5 | 5′-pTTTT/FAM/TGGATGGTGGTTGACGGCG-3′ | |
BFQ1b2 | 5′-TTGCCCCAGCAGGCGTTTT/BHQ1/TC-3′ | |
BFQ1b3 | 5′-TTGCCCCAGCAGGCGTTT/BHQ1/TTC-3′ | |
BFQ1b4 | 5′-TTGCCCCAGCAGGCGTT/BHQ1/TTTC-3′ | |
BFQ1b5 | 5′-TTGCCCCAGCAGGCGT/BHQ1/TTTTC-3′ | |
BFQ1b6 | 5′-TTGCCCCAGCAGGCG/BHQ1/TTTTTC-3′ | |
BFQ2 | BFQ2a2 | 5′-pC/VIC/TACGGCGTTTCACTTCTGAGTTCGGC-3′ |
BFQ2b3 | 5′-GGGAGACCCCACACTACCATCG/BHQ2/TCT-3′ | |
BFQ3 | BFQ3a2 | 5′-pT/ROX/TGCAGCAGCGGTCGGCAGCAGGTAT-3′ |
BFQ3b3 | 5′-TGGGCAGCGAGGAGCAGCA/BHQ2/TGC-3′ | |
Binary probes |  | 5′-/Cy3/ACCAATGGGCTCAGT-3′ |
|  | 5′-GAGTCCTTCC/Cy5/ACGAT-3′ | |
