Fig. 1: Developmental framework of auxin-mediated callus formation from scutellum in rice.
From: Initiation of scutellum-derived callus is regulated by an embryo-like developmental pathway in rice
a–d Tissue culture of rice mature seeds from wild-type (WT) (a), 35Spro:OsMIR393a (b), 35Spro:OsMIR393b (c), and Ostir1 Osafb2 (d) on CIM for 20 d. We tested more than 90 wild-type seeds, and all of them formed scutellum-derived callus. We tested more than 30 seeds from 35Spro:OsMIR393a, 35Spro:OsMIR393b, or Ostir1 Osafb2, and none of them formed scutellum-derived callus. e–i Sections showing callus formation from scutellum (e) of wild-type rice seeds on CIM at t0 (f), 2 d (g), 5 d (e, h), and 7 d (i). See Supplementary Fig. 2 for details. j–o Sections showing cell division from scutellum of 35Spro:OsMIR393b (j–l) and Ostir1 Osafb2 (m–o) seeds on CIM at t0 (j, m), 2 d (k, n), and 7 d (l, o). Scale bars, 5 mm (a–d), 500 μm (e), 50 μm (f–o).
