Fig. 5: OsLEC1 is involved in scutellum-derived callus formation.
From: Initiation of scutellum-derived callus is regulated by an embryo-like developmental pathway in rice
a–f Rice seeds from wild-type (a, d), Oslec1-1 (b, e), and Oslec1-2 (c, f) tissue-cultured on CIM for 26 d. g Statistical analysis of scutellum-derived callus formation in wild-type, Oslec1-1, and Oslec1-2 on CIM at 16 d. Error bars show SEM with nine biological replicates (10 calli in each replicate). The individual values are indicated by dots. **P < 0.01 in two-sided Student’s t-test compared with the wild-type control. h–j Sections showing cell division of scutellum epidermis from wild-type (h), Oslec1-1 (i) and Oslec1-2 (j) seeds on CIM at 7 d. k RT-PCR analysis of OsLEC1 expression pattern in vasculature-derived callus and scutellum-derived callus (33 cycles). OsUBQ5 serves as the control (30 cycles). Numbers indicate the days cultured on CIM. The bands of OsUBQ5 and OsLEC1 are derived from different gels. The bands from vasculature-derived callus and scutellum-derived callus were separately collected from the same gel, and the experiments were performed under the same conditions. The predicted band sizes were indicated. Two biological repeats were analyzed and showed the same result. l Primary roots of Oslec1-1 tissue-cultured on CIM for 20 d, showing vasculature-derived callus formation. See Fig. 3c for the wild-type control. Scale bars, 1 cm (a–c), 5 mm (d–f), 50 μm (h–j), 1 mm (l).
