Table 1 Synthetic miRNA sequences including one mismatch single base (highlighted in bold) and oligonucleotide sequences used for miR-21 and miR-34a detection assay.
Sequence (5’–3’) | |
---|---|
miR-21 | UAGCUUAUCAGACUGAUGUUGA |
miR-21 1m | AAGCUUAUCAGACUGAUGUUGA |
miR-21 22m | UAGCUUAUCAGACUGAUGUUGU |
miR-34a | UGGCAGUGUCUUAGCUGGUUGU |
21-capture | NH2–C12-(TTTTTTTTTTTT)TCAACATCAGT |
Biotin-21-capture | Biotinylated–C12-(TTTTTTTTTTTT)TCAACATCAGT |
34a-capture | NH2–C12-(TTTTTTTTTTTT)ACAACCAGCTA |
21-reporter-Cy5 | CTGATAAGCTA-Cy5 |
34a-reporter-Cy5 | AGACACTGCCA-Cy5 |
34a-reporter-Alexa488 | AGACACTGCCA-Alexa488 |