Fig. 6: Analysis of histology sections shows thinning of the outer nuclear layer in Lipe−/− mice. | Communications Biology

Fig. 6: Analysis of histology sections shows thinning of the outer nuclear layer in Lipe−/− mice.

From: Forward genetic screening using fundus spot scale identifies an essential role for Lipe in murine retinal homeostasis

Fig. 6

Representative H&E images (20× magnification) of a Lipe+/+ control retina (a) and a Lipe−/− retina (b) demonstrate moderate thinning of the ONL at 10 months of age. The number of nuclei in the ONL (c) and the thickness of the ONL (d) were measured on histology sections at 300 μm intervals starting from the optic nerve head (ONH). Lipe−/− mice had a significantly thinner outer nuclear layer in comparison to Lipe+/+ controls based on both the nuclear count (c) and ONL thickness measurements (d). ONL counts and ONL thickness were obtained from Lipe+/+ (n = 5 eyes) and Lipe−/− (n = 5 eyes). Data at each point of measurement are presented as Mean ± SEM. Two-tailed Student’s t test. #p < 0.1, *p < 0.05; **p < 0.01. Scale bar = 100 μm.

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