Fig. 7: Lipe is expressed in the retina and RPE of C57BL/6J (B6J) mice.
a Western blot analysis of retina and RPE-choroid from Lipe+/+ and Lipe−/− mice included the following samples: 1. Testes (positive control, 40 μg), 2. Lipe+/+ retina #1 (30 μg), 3. Lipe+/+ retina #2 (30 μg), 4. Lipe+/+ RPE-choroid #1 (20 μg), 5. Lipe+/+ RPE-choroid #2 (20 μg), 6. Lipe−/− retina (40 μg), 7. Lipe−/− RPE-choroid (40 μg), 8. Lipe−/− testes (40 μg). Antibodies for beta-actin and GAPDH were used as loading controls. b RNAscope in C57BL/6J (B6J) retina using a probe for Lipe demonstrated a strong signal in the retina, particularly in the outer nuclear layer. There was also some signal in the inner nuclear layer and RPE. c A B6J testis was used as a positive control for the Lipe probe. d An Sfxn3 probe in B6J retina21 shows a very different staining pattern compared to the Lipe probe; the staining was strongest in the inner nuclear layers for the Sfxn3 probe. e A DapB-bacterial dihydrodipicolinate reductase probe was used as a negative control in B6J testis. Scale bars for b and d = 50 μm, Scale bars for c and e = 75 μm.
