Fig. 4: Signatures of ageing in haematopoietic stem and progenitor cells. | Communications Biology

Fig. 4: Signatures of ageing in haematopoietic stem and progenitor cells.

From: Single-cell gene and isoform expression analysis reveals signatures of ageing in haematopoietic stem and progenitor cells

Fig. 4

A Frequency of cells per cluster between young and aged mice. B, C UMAP showing Procr expressing cells (HSCs) in young and aged mice, respectively. D Differential expression for each cluster, based on short-read data. Data are presented as a heatmap of fold-change values, where blue is upregulated with age and red is downregulated with age. E Differential expression in the HSC cluster. F Upregulation of aged HSC gene expression signature (from Svendsen et al.26) in cells from the aged HSC cluster. G qPCR confirmation of Sult1a1 and Nupr1 upregulation in individual aged phenotypic (LSK Cd48− Cd150+ Cd34−) HSCs (*** indicates p-value < 0.001, ** indicates p-value < 0.005).

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