Fig. 2: Monensin affects mitochondrial oxygen consumption in HCC cells. | Communications Biology

Fig. 2: Monensin affects mitochondrial oxygen consumption in HCC cells.

From: Boosting intracellular sodium selectively kills hepatocarcinoma cells and induces hepatocellular carcinoma tumor shrinkage in mice

Fig. 2

a Oxygen fluxes of C1C7 cells and hepatocytes (HPs) incubated for 4 h with (M) or without 10 μM Monensin in DMEM ± Na+. prior (T1) and after (T2) the addition of the ATP synthase inhibitor oligomycin (n ≥ 3 independent experiments). b Oxygen consumption linked to ATP production (T1-T2) of C1C7 cells and hepatocytes (HPs) incubated 4 h with or without 10 μM Monensin in DMEM ± Na+. Data are expressed as oxygen consumption normalized to the sample protein content (n ≥ 3 independent experiments). c Extracellular pH of C1C7 cells and HPs incubated 4 h with or without 10 μM Monensin in DMEM ± Na+ (n = 8 independent experiments). d Lactic acid release of C1C7 cells and HPs incubated 4 h with or without 10 μM Monensin in DMEM ± Na+ (n = 8 independent experiments). Values normalized to sample protein content (c, d) and expressed as % of control values (d). *P < 0.05, ***P < 0.001 with unpaired T-test.

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