Fig. 3: Effects of Monensin on Na⁺/K⁺ ATPase activity and of Na⁺/K⁺ ATPase inhibition.

a Basal activity Na⁺/K⁺ ATPase in Hepatocytes (HPs), C1C7 and HEPG2 cells exposed 1 h and 20 h to Monensin 10 μM (M) in DMEM ± Na⁺, evaluated as in nmol phosphate/min/mg protein and expressed as % of control values (n = 4 independent experiments). b Functional Na⁺ /K⁺ ATPase activity expressed as % difference of the Rubidium uptake, on respect to the control, for C1C7 cells incubated in Krebs buffer with 10 μM Monensin (n ≥ 2 independent experiments). c Cell viability of C1C7 cells and of HPs incubated with 10 μM Monensin (M) plus or minus 1 mM ouabain (ouab) Krebs ±  Na⁺. Results are expressed as % of respective controls value (n = 8 independent experiments). Symbols or bars represent the average while error bars represent the standard deviation. ***P < 0.001 with unpaired T-test.