Fig. 5: Monensin increases mitochondrial Na+ content and polarization. | Communications Biology

Fig. 5: Monensin increases mitochondrial Na+ content and polarization.

From: Boosting intracellular sodium selectively kills hepatocarcinoma cells and induces hepatocellular carcinoma tumor shrinkage in mice

Fig. 5

a Representative overlay confocal images of intracellular Na+ (Green: ING) and viable mitochondria (Red: MitoTrack). Cell nuclea were highlighted with DAPI (BLU). b Mitochondrial membrane polarization (JC1-1 red/green fluorescence) of C1C7 cell exposed (M) or not (C) 4 h to Monensin 10 μM in DMEM ± Na+ (n = 8 independent experiments).**P < 0.01, ***P < 0.001 with unpaired T-test. c, d Cellular water molecule efflux rate constants (kio) determined on HCC cells exposed (M) or not (C) to 10 μM Monensin up to 45 min in DMEM + Na+ (panel c, C1C7 on the left and HepG2 on the right) or Krebs ± Na+ (C1C7 cells, panel d) (c, d: n ≥ 3 independent experiments). Bars represent the average while error bars represent the standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001 with the Student’s t test.

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