Table 1 Kinetic parameters of GHs in Baccell WH2, BT and Bi. breve UCC2003 acting different β-glucan substrates and gluco-oligosaccharides.
From: Fungal β-glucan-facilitated cross-feeding activities between Bacteroides and Bifidobacterium species
Substrate | Kcat/Km (mg/ml/min) | kcat/Km (μM/min) | |
|---|---|---|---|
Baccell WH2 | |||
BcellWH2_01926 (GH3) | β-(1,6)glucobiose | 10.2 ± 0.9 | |
β-(1,6)glucotriose | 25.2 ± 1.8 | ||
BcellWH2_01931 (GH157) | Fusarium β-glucan | 1069 ± 11 | |
Linear β-(1,3)glucan | 2611 ± 38 | ||
Pustulan | Not detectable | ||
BcellWH2_02537 (GH30_3) | Fusarium β-glucan | 2512 ± 28 | |
Pustulan | 1968 ± 17 | ||
Linear β-(1,3)glucan | Not detectable | ||
β-(1,6)glucotriose | 2.5 ± 0.1 | ||
β-(1,6)glucohexaose | 9.8 ± 0.9 | ||
BcellWH2_02541 (GH2) | β-(1,3)glucobiose | 18.1 ± 1.2 | |
β-(1,3)glucotriose | 35.1 ± 2.9 | ||
BT | |||
BT3312 (GH30_3) | Fusarium β-glucan | 2874 ± 35 | |
Pustulana | 1776 ± 20 | ||
Linear β-(1,3)glucan | Not detectable | ||
β-(1,6)glucotriosea | 1.7 ± 0.1 | ||
β-(1,6)glucohexaosea | 6.0 ± 0.6 | ||
BT3314 (GH3) | β-(1,6)glucobiosea | 5.6 ± 0.2 | |
β-(1,6)glucotriosea | 6.7 ± 0.6 | ||
Bi. breve UCC2003 | |||
Bbr_0109 (GH1) | β-(1,3)glucobiose | 12.7 ± 1.8 | |
β-(1,4)glucobiose | 8.1 ± 0.9 | ||
β-(1,6)glucobiose | 5.4 ± 0.6 | ||
