Fig. 2: CPT1A promotes the growth and proliferation of ovarian cancer cells by regulating mitochondrial dynamics.

a Western blot analysis of MFF and β-tubulin in SKOV-3 and OVCAR-3 cells. Relative MFF expression was normalized to β-tubulin and then compared to control groups. b The EdU proliferation assay was performed in SKOV-3 and OVCAR-3 cells with control or MFF knockdown. Representative images (left) and the ratio of EdU-positive SKOV-3 and OVCAR-3 cells (right) are shown, n = 3 for each group. c Colony formation assay was performed in SKOV-3 and OVCAR-3 cells with control and MFF knockdown. Microphotographs covering representative areas of each treatment are shown (upper). The number of colonies in each case was analyzed (n = 3 for each group, lower). Scale bars, 2 mm. d Cell proliferation was determined by EdU assay in SKOV-3 and OVCAR-3 cells with control, CPT1A knockdown and exogenous overexpression of MFF in conjunction with CPT1A knockdown. Representative images (upper) and the ratio of EdU-positive (azide-555, red) SKOV-3 cells (lower) are shown, n = 3 for each group. e Cells described in (d) were seeded in 24-well plates and harvested for counting by the trypan blue assay for up to 7 days, n = 3 for each group. f ATP content detection in SKOV-3 cells with control, CPT1A knockdown, or MFF or CPT1A-WT overexpression in conjunction with CPT1A knockdown, n = 3 for each group. g The oxygen consumption rate (OCR) assay was performed in SKOV-3 cells with control, CPT1A knockdown, MFF knockdown or exogenous overexpression of MFF in conjunction with CPT1A knockdown. The relative OCR of maximal respiratory capacity (h) and reserved capacity (i) were quantified, n = 3 for each group. All data represent the mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001; n.s. indicates no significant difference compared with the control groups. Scale bars, 25 μm.