Fig. 1: Tumor-bearing mice exhibited impaired synaptic tagging and capture. | Communications Biology

Fig. 1: Tumor-bearing mice exhibited impaired synaptic tagging and capture.

From: Tumor-mediated microbiota alteration impairs synaptic tagging/capture in the hippocampal CA1 area via IL-1β production

Fig. 1

a Schematic illustration of murine HCC tumor model establishment. Mice were injected intrahepatic with either PBS or Hepa1-6 cells. Two weeks later, mice were killed, hippocampus was removed (Created with Biorender). b Schematic representation showing the location of electrodes in the CA1 region of the hippocampus. Recording electrode (rec) was positioned onto CA1 apical dendrites flanked by two stimulating electrodes S1 and S2 in the stratum radiatum (sr) to stimulate two independent Schaffer collateral (sc) synaptic inputs to the same neuronal populations. c After recording a stable baseline of 30 min, application of strong tetanization (STET) in S1 synaptic input (red circles) resulted in late-Long Term Potentiation (Late-LTP). The control potentials in S2 (blue circles) were relatively stable throughout the recording in Mock mice (n = 6). d After recording a stable baseline of 30 min, application of STET in S1 synaptic input (red circles) resulted in Late-LTP. The control potentials in S2 (blue circles) were relatively stable throughout the recording in tumor-bearing mice (n = 5). e STC was tested using strong before weak (SBW) paradigm. Induction of early-LTP in S2 (blue circles) by WTET 30 min after the Late-LTP induction in S1 (red circles) by STET resulted in Late-LTP in both the synaptic inputs, thereby expressing STC (n = 6). f The same SBW paradigm when used to test STC in tumor-induced mice failed to express STC (n = 6). All data represent mean ± SEM. Representative fEPSP traces shown in each case recorded at −30 min (solid line); 60 min (dotted line); and 180 min (hatched line). Single arrow represents the time point of application of WTET for the induction of early‐LTP. Triplet of arrows represent the point of application of STET for the induction of late‐LTP. Scale bars for all the traces, vertical: 3 mV; horizontal: 5 ms. sc Schaffer collaterals, sr stratum radiatum, mf mossy fibers, DG dentate gyrus. The experiments were repeated 3 times.

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