Fig. 7: JNK1-overexpression improves mitochondrial abundance for EAAT2 association.

a Representative SR-SIM immunofluorescence images of control and ALS ACs, demon- strating α-tubulin-, EAAT2-IR and mitoB-stained mitochondria. Insets (lower image panels) illustrate magnified areas indicated by the yellow boxes. b Plots illustrate the percentages of mitoB + /EAAT2+ area-over- laps in control and SOD1 ALS AC processes. n = 8 control and 8 SOD1 ALS ACs (5-6 images per AC from two experiments); Data represents mean ± SD. c Western blot (WB) images of EAAT2 immunoreactive bands in control and SOD1 ALS AC samples. Graph shows band densities normalised to control (1) and to β-actin bands. n = 3 independent batches of control and ALS AC cultures; data represent mean ± SEM. d Representative SR-SIM immunofluorescence images of non-or JNK1-GFP-transfected ALS AC processes, demonstrating a-tubulin + , EAAT2+ and mitoB-stained (mitochondria) objects. Insets (lower image panels) illustrate magnified areas indicated by the yellow boxes. e Graph illustrates area-overlaps between mitoB + /EAAT2+ immunoreactive objects in AC processes. n = 8 JNK1-GFP- and 10 JNK1-GFP+ ACs from two experiments (5-6 images per AC from two experiments); data expressed as mean ± SD. f WB images of COX IV and EAAT2 immunoreactive bands in GFP and JNK1-GFP vector transfected ALS AC samples. Graphs show band densities normalised to GFP (non-JNK1 + ) AC bands (1) and to b-actin bands. n = 3 independently transfected AC culture batches per group; data expressed as mean ± SEM. For each analysis, unpaired two-tailed t-test was used. Scale bars: Scale bars: 2μm (1μm for insets). Please, also see Suppl. Fig. 10.