Fig. 1: Bulk RNA-seq results of SUSD2- and SUSD2+ cells from myometrial samples.

a Representative flow cytometry scatter plot of single cell SUSD2- and SUSD2+ sort showing over 30% of the live myometrial cells positive for the SUSD2 marker. Boxed areas indicate gating strategy. b Principal component analysis (PCA) plot of RNA-seq results from SUSD2- (in black) and SUSD2+ (in red) cells, labels represent individual patient samples (n = 5), and variance for each PC is indicated in percentage. c Volcano plot showing up (n = 3527) and down (n = 3250) DEGs with an FDR-adjusted p-value < 0.05 in SUSD2+ vs. SUSD2- cells depicted as red dots. Gray dots represent genes with an FDR p-value > 0.05. d Boxplot of mesenchymal stem cells markers, SUSD2, MCAM, PDGFRβ and CSPG4 in the SUSD2- (in gray) and SUSD2+ (in red) cell population (n = 5). Gene expression is shown as log₂CPM. SUSD2+ sorted cells are significantly enriched for MSC markers including SUSD2 (log₂FC = 4.5, FDR p = 2.9 × 10⁻⁵), MCAM (log₂FC = 3.1, FDR p = 8.8 × 10⁻¹⁶), PDGFRβ (log₂FC = 2.8, FDR p = 1.5 × 10⁻¹¹) and CSPG4 (log₂FC = 3.4, FDR p = 1.1 × 10⁻¹¹). e Heatmap of the top 300 DEGs from SUSD2+ vs. SUSD2- cells comparison with unsupervised hierarchical clustering of genes and samples (n = 5). Color gradient represents gene expression as z-score.