Fig. 4: High-throughput screen (HTS) of regulators of the IR-induced RNA sensor pathway. | Communications Biology

Fig. 4: High-throughput screen (HTS) of regulators of the IR-induced RNA sensor pathway.

From: Transposable elements potentiate radiotherapy-induced cellular immune reactions via RIG-I-mediated virus-sensing pathways

Fig. 4

a Experimental design (left) and the results (right) of the HTS of the kinase library. At a concentration of 0.5 μM, 137 kinase inhibitors suppressed IRSE activity by at least 50%, with 32 suppressing IRSE activity by 80% or more. b ISRE activity in A549 cells at 96 h after treatment with the indicated inhibitors at the indicated concentrations. Data were representative of two independent experiments. c GO terms enriched in genes whose expression was induced by radiation (fold change >2) and suppressed by mTOR inhibition (fold change <0.5). d Expression of TEs in WT and mTOR-knockout (KO) IR cells and in WT NIR cells. Data were representative of biological replicates. The Z- score was calculated using the R package pheatmap from the TPM of the indicated TEs, and then clustering was performed using the same package.

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