Fig. 3: Mutations that introduce aromatic rings into the Hb (Z) binding pocket induce gate opening. | Communications Biology

Fig. 3: Mutations that introduce aromatic rings into the Hb (Z) binding pocket induce gate opening.

From: High resolution structures define divergent and convergent mechanisms of archaeal proteasome activation

Fig. 3

a Structure of ZYA-T20S with simulated mutation of A154F and V24F shown in yellow sticks with dotted clouds, showing overlap between these mutants and the Z group binding location (ZYA; colored in red). b 7 nM T20S (WT or V24F) incubated with 44 nM PA26 or 15 nM PAN (supplemented with ATP and MgCl2). LFP degradation rate (rfu/min) normalized to control and the amount of proteasome from different preparations is normalized to their rate of LLVY-AMC hydrolysis, which is insensitive to gating affects (see methods for details). c Same as b but 7 nM T20S (WT or A154F). LFP degradation rate (rfu/min) normalized as in b. d Same as b but 7 nM T20S (WT or V24Y). LFP degradation rate (rfu/min) normalized as in b. Data (means) are representative of three or more independent experiments each performed in triplicate. Error bars represent ± standard deviation.

Back to article page