Fig. 1: PAE increases neuronal ELOVL4 expression in the motor and cingulate cortices at P30.

a The number of ELOVL4-positive cells is higher in the motor cortex of PAE mice compared to the control mice (p = 0.038). ^*p < 0.05. Student’s t-test. Control n = 12, PAE n = 10. b Representative images for immunolabeling of ELOVL4 (red) and DAPI (blue). Scale bar = 10 µm. c Representative images of coronal section from control cortex demonstrate that ELOVL4 expression (red) is detected in NeuN-positive neurons (green). Scale bar = 25 µm. d The number of ELOVL4-positive cells is higher in the cingulate cortex of PAE mice compared with control mice at P30 (p = 0.011). ^*p < 0.05. Student’s t-test. Control n = 9, PAE n = 11. e Representative images of ELOVL4 (red) and DAPI (blue) staining in the cingulate cortex. Scale bar = 10 µm. f The number of ELOVL4-positive cells in the cerebellum is not different between control and PAE mice at P30. Control n = 4, PAE n = 5. g Representative images of ELOVL4 immunostaining in the cerebellum. Scale bar = 50 µm. Graphs represent mean ± SEM. Each dot represents an individual animal. GL granular layer, WM white matter.