Fig. 3: LDs are accumulated in various brain regions in PAE mice at P30.

a Highlighted areas in the schema indicate brain regions analyzed in mice. b The numbers of LD included cells were quantified in 200 × 200 µm2 area in each brain region. The data show an increase of LD accumulating cells in the motor cortex (p = 0.0352), striatum (p = 0.0311), both DG and CA3 of the hippocampus (p = 0.0267 and 0.0466, respectively), and lateral ventricle (LV) wall (p = 0.067) in PAE mice. *p < 0.05. Student’s t-test. Control n = 8; PAE n = 12. Graphs represent mean ± SEM. Each dot represents an individual animal. c Representative images of ORO stained brain areas show changes in the number of cells accumulating LDs. Insets show higher magnification views around red arrowheads or white square of each panel. Scale bar = 20 µm. Arrowheads indicate LDs.