Fig. 1: SERF1a enhances fibrillization of wild-type and mutant TrxHtt exon 1. | Communications Biology

Fig. 1: SERF1a enhances fibrillization of wild-type and mutant TrxHtt exon 1.

From: Amyloid modifier SERF1a interacts with polyQ-expanded huntingtin-exon 1 via helical interactions and exacerbates polyQ-induced toxicity

Fig. 1

a ThT fluorescence assay of TrxHtt exon 1 in the absence or presence of SERF1a. Fifty μM of different polyQ-expanded Htt exon 1 proteins, including TrxHttex1-15Q, TrxHttex1-29Q, TrxHttex1-39Q, and TrxHttex1-49Q, were incubated at 37 °C with continuous shaking at 200 rpm with and without equimolar SERF1a. n = 3. The error bars represent standard deviation. b Filter retardation assay of the end-point products of ThT assay after incubation. More aggregates were found in TrxHttex1 with SERF1a. c TEM images of the end-products of ThT assay. The scale bars are 500 nm. d Dot blot of TrxHtt exon 1 variants with and without SERF1a at different incubation times. TrxHttex1 proteins were detected by 1C2 and MW7 antibodies. e Far-UV CD analysis of TrxHttex1 variants with and without SERF1a at different incubation times. Buffer background was subtracted from the spectra of TrxHttex1 alone, and SERF1a alone spectra were subtracted from that of TrxHttex1 with SERF1a. f The ellipticity at 216 nm from the far-UV spectra of panel (e) was plotted.

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