Fig. 2: SERF1a interacts with Htt exon 1. | Communications Biology

Fig. 2: SERF1a interacts with Htt exon 1.

From: Amyloid modifier SERF1a interacts with polyQ-expanded huntingtin-exon 1 via helical interactions and exacerbates polyQ-induced toxicity

Fig. 2

Htt exon 1 variants at 25 μM was titrated with SERF1a. Intrinsic fluorescence was monitored at an excitation of 257 nm and an emission of 282 nm. Signals were normalized to the starting signal. a Fluorescence quenching of TrxHttex1 variants upon titration with SERF1a. b Fluorescence quenching of Httex1-15Q and Httex1-39Q without thioredoxin tag upon titration with SERF1a. c Fluorescence quenching of TrxHttex1-15Q and TrxHttex1-49Q in the absence or presence of 5% TFE upon SERF1a titration.

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