Fig. 5: SERF1a induces conformational transition of TrxHttex1-49Q to a more homogeneous species. | Communications Biology

Fig. 5: SERF1a induces conformational transition of TrxHttex1-49Q to a more homogeneous species.

From: Amyloid modifier SERF1a interacts with polyQ-expanded huntingtin-exon 1 via helical interactions and exacerbates polyQ-induced toxicity

Fig. 5

TrxHttex1-49Q was incubated with and without equimolar SERF1a, and time-course samples were collected for experiments. a Analytical ultracentrifugation analysis of TrxHttex1-49Q at different incubation times. TrxHttex1-49Q with and without SERF1a was incubated, and samples at 0, 6, 12, 24, and 48 h were collected and subjected to sedimentation velocity (SV) experiments. Data were analyzed in a continuous c(s) distribution model, and sedimentation coefficients were obtained. b Size-exclusion chromatography (SEC) of TrxHttex1-49Q in the absence or presence of SERF1a. TrxHttex1-49Q was incubated with and without equimolar SERF1a, and time-course samples were collected at different timepoints for SEC. Peaks were labeled with different symbols #, §, and *. c Native PAGE for SEC fractions of TrxHttex1-49Q with and without SERF1a. Fractions 14 and 17 from SEC at different timepoints were collected for native PAGE and detected by silver staining. d Dot blot for SEC fraction 14 and 17 probed with SERF#1 (1:100). rSERF1a: recombinant SERF1a as a positive control. e Far-UV CD analysis for SEC fraction 14 and 17. Buffer background was subtracted from the spectra of fraction 14, and SERF1a alone spectra were subtracted from that of fraction 17.

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